Effect of probiotics on the reduction of streptococcus mutans in pediatric patients.
DOI:
https://doi.org/10.47990/4zd6cr10Keywords:
Bacterioterapia, Probiótico, Disbiosis, Caries, Streptococcus mutansAbstract
Introduction: In Pediatric Dentistry, probiotics represent a new area of research focused on Bacteriotherapy. These probiotics modulate the viability and adhesion of cariogenic bacteria by competing for adhesion on dental surfaces and modifying the surrounding environment, which helps to modulate pH. Objective: To compare the effect of short-term consumption of two strains of probiotics on the count of colony-forming units of Streptococcus mutans, as well as to assess the change in salivary pH using reagent strips in pediatric patients in an in vitro study. Materials and Methods: Two strains of probiotics were administered to 10 children aged 6-12 years with caries, randomly assigned to two groups. One group consumed a probiotic tablet (Group I), while the other group took 6 drops daily (Group II) for 28 days. pH and Streptococcus mutans counts were recorded on days 0, 15, and 28, and statistically compared using Friedman and Dunn tests. Results: A significant reduction in Streptococcus mutans was observed with the application of Lactobacillus rhamnosus LGG with Lactobacillus acidophilus (p<0.0239) between day 0 and 15, along with changes in salivary pH (p<0.0008), compared to Bifidobacterium lactis BB-12 with Lactobacillus rhamnosus LGG (p<0.0085) between day 0 and 29, with no observed changes in salivary pH. Conclusions: The combination of probiotics (Bifidobacterium lactis BB-12 with Lactobacillus rhamnosus LGG) significantly reduced Streptococcus mutans in the short term, while the combination (Lactobacillus rhamnosus LGG with Lactobacillus acidophilus) helped neutralize salivary pH parameters.
References
Çaglar E, Kargul B, Tanboga I. Bacteriotherapy and probiotics’ role on oral health. Oral Dis [Internet]. 2005;11(3):131–7. Disponible en: http://dx.doi.org/10.1111/j.1601-0825.2005.01109.x
Ricketts D, Lamont T, Innes NPT, Kidd E, Clarkson JE. Operative caries management in adults and children. Cochrane Libr [Internet]. 2013; Disponible en: http://dx.doi.org/10.1002/14651858.cd003808.pub3
Cagetti M, Mastroberardino S, Milia E, Cocco F, Lingström P, Campus G. The use of probiotic strains in caries prevention: A systematic review. Nutrients [Internet]. 2013 [citado el 20 de marzo de 2024];5(7):2530–50. Disponible en: https://www.mdpi.com/2072-6643/5/7/2530
Twetman S. Are we ready for caries prevention through bacteriotherapy? Braz Oral Res [Internet]. 2012 [citado el 19 de marzo de 2024];26(spe1):64–70. Disponible en: https://www.scielo.br/j/bor/a/F4Gj7pMwtbKq6RYZm7XY3tB/?lang=en
Fierro-Monti C, Aguayo-Saldías C, Lillo-Climent F, Riveros-Figueroa F. Rol de los Probióticos como Bacterioterapia en Odontología. Revisión de la Literatura. Odontoestomatologia [Internet]. 2017;19(30):4–13. Disponible en: http://dx.doi.org/10.22592/ode2017n30a2
Faujdar SS, Mehrishi P, Bishnoi S, Sharma A. Role of Probiotics in human health and disease: An update. Int J Curr Microbiol Appl Sci [Internet]. 2016;5(3):328–44. Disponible en: https://d1wqtxts1xzle7.cloudfront.net/67657457/Sameer_20Singh_20Faujdar__20et_20al-libre.pdf?1623942906=&response-content-disposition=inline%3B+filename%3DRole_of_Probiotics_in_human_health_and_d.pdf&Expires
Meurman JH, Stamatova IV. Probiotics: Evidence of oral health implications. Folia Med (Plovdiv) [Internet]. 2018;60(1):21–9. Disponible en: http://archive.sciendo.com/FOLMED/folmed.2017.60.issue-1/folmed-2017-0080/folmed-2017-0080.pdf
Allaker RP, Ian Douglas CW. Non-conventional therapeutics for oral infections. Virulence [Internet]. 2015;6(3):196–207. Disponible en: http://dx.doi.org/10.4161/21505594.2014.983783
Wasfi R, Abd El-Rahman OA, Zafer MM, Ashour HM. Probiotic Lactobacillus sp. inhibit growth, biofilm formation and gene expression of caries‐inducing Streptococcus mutans. J Cell Mol Med [Internet]. 2018;22(3):1972–83. Disponible en: http://dx.doi.org/10.1111/jcmm.13496
Näse L, Hatakka K, Savilahti E, Saxelin M, Pönkä A, Poussa T, et al. Effect of long-term consumption of a probiotic bacterium, Lactobacillus rhamnosusGG, in milk on dental caries and caries risk in children [Internet]. Ilele.hk. 2001 [cited 2024 Mar 5]. Available from: http://pc.ilele.hk/public/pdf/20190528/7a37d824f4d8e0056eb396b77ca9845c.pdf
Jindal G, Pandey RK, Agarwal J, Singh M. A comparative evaluation of probiotics on salivary mutans streptococci counts in Indian children. Eur Arch Paediatr Dent [Internet]. 2011;12(4):211–5. Disponible en: http://dx.doi.org/10.1007/bf03262809
Hasslöf P, Stecksén-Blicks C. Chapter 10: Probiotic Bacteria and Dental Caries. En: Monographs in Oral Science. S. Karger AG; 2020. p. 99–107.
Haukioja A. Probiotics and oral health. Eur J Dent [Internet]. 2010;04(03):348–55. Disponible en: http://dx.doi.org/10.1055/s-0039-1697851
Sivamaruthi BS, Kesika P, Chaiyasut C. A review of the role of probiotic supplementation in dental caries. Probiotics Antimicrob Proteins [Internet]. 2020;12(4):1300–9. Available from: http://dx.doi.org/10.1007/s12602-020-09652-9
Çaglar E, Onder Kuscu O, Selvi Kuvvetli S, Kavaloglu Cildir S, Sandalli N, Twetman S. Short-term effect of ice-cream containingBifidobacterium lactisBb-12 on the number of salivary mutans streptococci and lactobacilli. Acta Odontol Scand [Internet]. 2008;66(3):154–8. Available from: http://dx.doi.org/10.1080/00016350802089467
Villavicencio J, Villegas LM, Arango MC, Arias S, Triana F. Effects of a food enriched with probiotics on Streptococcus mutans and Lactobacillus spp. salivary counts in preschool children: a cluster randomized trial. J Appl Oral Sci [Internet]. 2018;26(0). Available from: http://dx.doi.org/10.1590/1678-7757-2017-0318
Pinto GS, Cenci MS, Azevedo MS, Epifanio M, Jones MH. Effect of yogurt containing Bifidobacteriumanimalis subsp. Lactis DN-173010 probiotic on dental plaque and saliva in orthodontic patients. Caries Res [Internet]. 2014 [citado el 20 de marzo de 2024];48(1):63–8. Disponible en: https://karger.com/cre/article-abstract/48/1/63/86087/Effect-of-Yogurt-Containing?redirectedFrom=fulltext
Taipale T, Pienihäkkinen K, Salminen S, Jokela J, Söderling E. Bifidobacteriumanimalis subsp. lactis BB-12 administration in early childhood: A randomized clinical trial of effects on oral colonization by mutans streptococci and the probiotic. Caries Res [Internet]. 2012 [citado el 20 de marzo de 2024];46(1):69–77. Disponible en: https://karger.com/cre/articleabstract/46/1/69/85812/Bifidobacteriumanimalis-subsp-lactis-BB-12
Shivangi S, Devi PB, Ragul K, Shetty PH. Probiotic potential of Bacillus strains isolated from an acidic fermented food idli. Probiotics Antimicrob Proteins [Internet]. 2020;12(4):1502–13. Available from: http://dx.doi.org/10.1007/s12602-020-09650-x
Downloads
Published
Issue
Section
License
Copyright (c) 2025 Latin American Pediatric Dentistry Journal
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
How to Cite
